在生醫晶片中，蛋白質吸附(Protein Adsorption)的現象是影響疾病檢測(Diseases Diagnosis)的重要考量，因為蛋白質吸附牽涉到反應分子的效能。本研究數值模擬了解不同入口速度、入口方式、長度及截面積之微流道(Microfluidic Channel)下，計算有多少生醫分子經過微流道，觸碰到壁面而被吸附，藉此了解流道設計與蛋白質吸附之關係。
利用微铣削機(Micro Milling machine)加工PMMA基材製造微流道模具，再利用PDMS翻印出微流道形狀，使用氬氣常壓電漿改質PDMS微流道表面最後與玻璃作黏合完成微流道晶片製作。
為了驗證模擬之結果，利用多次實驗來討論生醫分子的吸附性。實驗中之生醫分子為牛血清蛋白(BSA)，利用Nanoorange螢光標示物標記牛血清蛋白。在實驗中將螢光檢體流經微流道晶片，使用共軛焦顯微鏡(Confocal)與螢光顯微鏡來觀察量測蛋白在微流道的吸附情形，並拍攝影像進行定量分析。模擬的結果了解蛋白質吸附和微流道的設計有相當之關係，但礙於多次實驗的不穩定性，無法直接驗證模擬之結果。

The protein adsorption is a critical factor in influencing the performance of a biochip which is used for disease detection. In this study, numerical simulation was used to estimate the protein adsorption rate in terms of the flowing velocity, design of the inlet, the length of the microchannel.
To validate the simulation results, the microfluidic devise was fabricated with a micromilling machine and the PDMS casting technique. Then the microchannel was sealed with another piece of glass with the surface plasma treatment and used for experiments.
Multiple experiments were conducted either with a confocal microscope or a fluorescence microscope, but the results were not steady enough to compare or even conclude with the simulation result. Therefore modification of the experiments will be made to improve the quality of the experiments, then validate the simulation results.

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