本研究利用臭氧對聚碸 (polysulfone, PSF) 中空纖維膜進行處理，使薄膜表面產生過氧化基，進一步與丙烯酸單體產生自由基聚合反應，再使用EDC活化表面之羧基，最後將唾液酸(sialic acid)分子固定於中空纖維膜表面。
改質後的中空纖維膜利用 (1,1-diphenyl-2-picrylhydrazyl, DPPH) 測試臭氧濃度及其處理時間的最佳化條件; 利用重量法測試表面羧基密度; 使用 (Thiobarbituric acid assay) TBA測試固定於PSF中空纖維膜上的唾液酸含量。由SEM和AFM觀察改質前後是否對中空纖維膜上的孔洞及表面粗糙度造成影響。以凝血時間探討改質前後是否影響其血液相容性。另一方面，以改質前後之中空纖維膜進行脂多醣吸附評估以及清除活性氧之測試。
結果表示，經各階段的處理過程，並未造成改質之中空纖維膜顯著地型態改變; 也不影響到原本中空纖維膜的凝血性質。此外，改質後的中空纖維膜對於吸附脂多醣的能力明顯地提升; 且對於清除活性氧之能力大大提高。
由全部結果顯示出，經改質後的中空纖維膜對於吸附脂多醣以及清除活性氧的能力顯著，且對材料型態及其血液相容性不構成顯著地影響。

Polysulfone hollow fiber membrane was treated with ozone to introduce peroxide and futher initiated free radical polymerization of acrylic acid. The resulting carboxyl groups were activated with EDC to covalently immobilize sialic acid. The conditions for ozone treatment was determined by measuring the surface concentration of peroxide with DPPH. The surface concentration of carboxyl groups was determined by weighing method. The surface concentration of sialic acid was determined using TBA test. The porous structure and surface roughness was examined using SEM and AFM. The hemocompatibility of the resulting hollow fiber was evaluated based on the blood coagulation time. In addition, the clearances of lipopolysaccharide (LPS) and reactive oxygen species (ROS) were also measured. The results show that the modification incurred little affect on the morphology and the blood coagulation performance. However, the adsorptibility of LPS and the clearance toward ROS were greatly increased after the immobilization of sialic acid.

財團法人中華民國腎臟基金會(http://www.kidney.org.tw/)
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