本研究方向主要區分為兩大部分，第一部份主要是探討能提高菌體氧氣利用能力之透明顫菌血紅蛋白（Vitreoscilla hemoglobin；VHb）對木質醋酸菌 ( Acetobacter xylinum；A. xylinum)轉型株之影響。首先以pBBR122 載體進行A. xylinum電轉殖與化學轉殖之測試，結果電轉殖法有較高的轉殖效率。在轉殖方法成功建立後，我們將VHb基因轉殖入 A. xylinum 菌體中，並開始探討A. xylinum轉型株在充氧和微氧的條件下，菌體生長及生產細菌纖維素之研究。實驗結果顯示VHb基因表現可幫助 A. xylinum 菌體的生長，在充氧的條件下，含VHb基因表現的重組菌株比生長速率為宿主細胞的3倍；在微氧的條件下含VHb基因表現的重組菌株比生長速率則和宿主細胞相當。在探討細菌纖維素的研究方面，在充氧的條件下含VHb基因表現的重組菌株細菌纖維素的生產量為宿主細胞的2倍，在微氧的條件下含VHb基因表現的重組菌株細菌纖維素的生產量為宿主細胞的3倍。

第二部分研究是延續第一部分的結果以A. xylinum菌體為主軸，探討是否能夠藉由在A. xylinum中本身就有UDP-Glucose dehydrogenase (aceM) 和UDP-Glucose pyrophorylase (hasC)，我們再將hyaluronic acid synthase (hasA)基因轉殖入 A. xylinum 菌體中，希望能夠利用A. xylinum合成透明質酸。結果顯示我們成功的將hyaluronic acid synthase (hasA)基因轉殖入 A. xylinum 菌體中進行表現，但是由於hyaluronic acid synthase(hasA)是膜蛋白，表現量相當的少，所以我們只針對透明質酸產量做初步檢測，結果發現利用 A. xylinum合成透明質酸是可行的。

Bacterial cellulose can be produced by Gram-negative bacterium,such as rod-shaped Acetobacter xylinum. As the organism is obligate aerobic, bacterial cellulose is always produced at the air/liquid interface. In a static culture, the oxygen supply is considered as the limiting factor for cell growth and cellulose production. Vitreoscilla, a strict aerobic bacterium, synthesizes bacterial hemoglobin (VHb) to survive and grow in an O2-poor environment.VHb plays the role in facilitating oxygen transfer to the respiratory chain in cell membrane. In this work, the constitutive bla-promoter was employed to drive the expression of VHb in cellulose producing bacteria, Acetobacter xylinum BCRC12334.The feasibilities of using bla-promoter in Acetobacter xylinum to drive expression of heterologous proteins as well as the function of expressed VHb to enhance the cell growth and cellulose productivity at microaerobic and anaerobic condition were studied. The results show that VHb did improve cellulose yield during the static flask cultivation at microaerobic and anaerobic condition. The growth rate of VHb expressing strain VHb+ was 3 fold higher than that of host strain at microaerobic condition. At anaerobic condition, the growth rate of VHb+ strain also similar to that of host strain. The amount of bacterial cellulose producted by VHb+ strain was 2 fold higher than that of host strain under the microaerobic condition. At anaerobic condition, the produced amount of bacterial cellulose by VHb+ strain was 3 fold of that produced by host strain.

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