研究生: |
Satita Gerdsapaya Satita Gerdsapaya |
---|---|
論文名稱: |
Au@PPy Core/Shell Nanoparticle for Sensitive EC-SERS Based Determination of Cortisol in Saliva Au@PPy Core/Shell Nanoparticle for Sensitive EC-SERS Based Determination of Cortisol in Saliva |
指導教授: |
蘇威年
Wei-Nien Su 黃炳照 Bing-Joe Hwang |
口試委員: |
蘇威年
Wei-Nien Su 黃炳照 Bing-Joe Hwang 周澤川 Tse-Chuan Chou 蔡孟哲 Meng-Che Tsai |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 應用科技研究所 Graduate Institute of Applied Science and Technology |
論文出版年: | 2020 |
畢業學年度: | 108 |
語文別: | 英文 |
論文頁數: | 112 |
中文關鍵詞: | SERS 、EC-SERS 、Au@PPy core/shell nanoparticles 、Au@PPy-CMab nanoparticles 、Immunosensor 、Rhodamine 6G (R6G) 、Cortisol 、Saliva |
外文關鍵詞: | SERS, EC-SERS, Au@PPy core/shell nanoparticles, Au@PPy-CMab nanoparticles, Immunosensor, Rhodamine 6G (R6G), Cortisol, Saliva |
相關次數: | 點閱:223 下載:0 |
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皮質醇普遍稱為逆境激素,其變異或異常被歸因於疾病與綜合症狀,因此皮質醇檢測對於篩檢與監測各種健康狀況是極為重要的。我們的工作使用電化學表面增強拉曼散射(EC-SERS)法開發Au @ PPy核/殼NP,用於檢測唾液中的皮質醇。AuNPs運用R6G於評估SERS的增強因子,其塗佈於平均大小為46nm的PPy殼厚度,其PPy殼厚度取決於Py的聚合時間與單體體積。Au @ PPy(〜46 nm核心/〜1 nm外殼厚度)可獲得的最高增強因子高達1.74 x 106。我們選擇其條件去結合抗體(CMab)與EC-SERS的應用。 EC-SERS探針源自抗體-抗原相互作用,從而增加了皮質醇檢測的選擇性與靈敏度,結果指出使用 EC-SERS法的Au @ PPy-CMab可在有限且寬廣的放範圍下6.69 x 10-12 M偵測皮質醇,並可用於檢測人體唾液中的皮質醇。我們的工作提出SERS探針的快速製造法,且可應用於天然基質中監測疾病與綜合症狀進展的感測方法。
Cortisol is commonly called the stress hormone. Its variations or abnormalities might give rise to diseases and syndromes. Thus, detecting cortisol with high sensitivity and confidence is very important for diagnosis and in monitoring various health conditions. This work is to develop Au@PPy core/shell NPs with the electrochemical surface-enhancement Raman scattering (EC-SERS) methods for the detection of cortisol in saliva. The synthesized AuNPs with an average size of ~46 nm, coated with various PPy (polypyrrole) shell thickness by varying polymerization time and volume of Py (pyrrole) monomer, are used to evaluate the SERS enhancement factor with R6G. The highest enhancement factor was up to 1.74 x 106 attained with Au@PPy (~46 nm core/~1 nm shell thickness). The coated nanoparticles were conjugated with antibody (CMab) and further applied for EC-SERS. The interaction between antibody-antigen increases the selectivity and sensitivity of the EC-SERS probes for detecting the cortisol. Cortisol can be successfully detected in a wide range of concentrations by the application of Au@PPy-CMab and EC-SERS method, with a limit of detection (LOD) down to 6.69 x 10-12 M. Cortisol levels in human saliva were also tested for verification. Our work presents a rapid fabrication of SERS probes and can be applied as sensor devices for monitoring disease and syndromes progression in natural matrices.
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